reform provides a fast, easy, reliable, and reproducible solution for creating modified reference genome data for use with standard analysis pipelines and the 10x Cell Ranger pipeline.

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Email Address When job is complete this e-mail will receive the download links
Chromosome ID of the chromosome to modify. Must match ID in FASTA file.
Reference Sequence (FASTA) URL to reference FASTA file. e.g. ftp://ftp.ensembl.org/pub/release-88/fasta/mus_musculus/dna/Mus_musculus.GRCm38.dna.toplevel.fa.gz
Reference Annotation (gff3 or gtf) URL to reference gff file. e.g. ftp://ftp.ensembl.org/pub/release-88/gff3/mus_musculus/Mus_musculus.GRCm38.88.gff3.gz
Inserted Sequence (FASTA) New sequence to be inserted into reference genome.	Inserted Reference (gff3 or gtf) GFF file describing new FASTA sequence to be inserted.

NOTE: Either position, or upstream AND downstream sequence must be provided.	Position Position in chromosome at which to insert <in_fasta>. Can use -1 to add to end of chromosome. Note: Position is 0-based
	Upstream Sequence FASTA file with upstream sequence.	Downstream Sequence FASTA file with downstream sequence.